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Appendix D - Forum
D.3 - Library Preparation
5'-peak in read coverage
Anchored primers for RT
Error at library preparation step
How do I modify the PCR amplification protocol?
New error at library preparation step
Noise while preparing library
Source of strong bias towards 5' ends
Understanding behaviour of the RT_MOTIF simulation parameter
What paramters should I change if I would like to observe strong GC bias?
What paramters should I use to create a strong positional (3') bias?
Why do I obtain reads that are shorter than my specified READ_LENGTH in the .PAR file?
Why no fragmentation by sonication?
Why simulating fragment lengths obtained by hydrolysis by Weibull distributions?
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Appendix D - Forum
D.3 - Library Preparation
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