The format of LIB (Simulated Library) files is simple and condenses the information needed to describe a fragment (RNA or cDNA) of an original transcripts. Each line corresponds to one such fragments and in 3 tab-delimited fields the estart, eend in the spliced sequence (exonic) of the transcript with transcript_id of the original annotation.
| Nr | Name | Type | Description |
|---|---|---|---|
| 1 | estart | Integer | exonic start and end position, 0-based. Values larger than the transcript length indicate positions in the poly-A tail, negative values are caused by variations of the transcription start site to upstream positions. |
| 2 | eend | Integer | |
| 3 | transcript identifier | String | an string uniquely identifying the transcript. As by UCSC, the prefix of the reference sequence name is joined by an '@' symbol |
| 4 | number of copies | Integer | the number of identical copies of this fragment |
Example
1456 1686 chr1:4847775-4887990W@NM_011541 0 2238 2441 chr1:4847775-4887990W@NM_011541 0 116 290 chr1:4847775-4887990W@NM_011541 0 199 423 chr1:4847775-4887990W@NM_011541 0 1510 1711 chr1:4847775-4887990W@NM_011541 0 1196 1361 chr1:4847775-4887990W@NM_011541 0 1669 1875 chr1:4847775-4887990W@NM_011541 0 270 480 chr1:4847775-4887990W@NM_011541 0
Note: because the simuated transcription start and length of the poly-A tail may vary from the annotation in the reference, values for estart can drop below , and values for eend can take values higher than the transcript length.
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