Parameters

Parameter

Name

Default

Value

Description
LIB_FILE_NAME
 input file that contains the set of fragments from which reads are sampled
SEQ_FILE_NAME
 output file where genomic positions of sequenced reads are stored to
READ_NUMBER5,000,000number of reads sequenced
READ_LENGTH36length of the reads
PAIRED_ENDfalseswitch on/off paired-end reads

Overview

During the "Final Library Preparation" step of the Flux Simulator pipeline the cDNA fragments are optionally filtered by gel segregation, and also optionally amplified by a PCR (i.e., polymerase chain reaction).

Details

  • No labels