Parameter Name | Default Value | Description |
|---|---|---|
LIB_FILE_NAME | input file that contains the set of fragments from which reads are sampled | |
SEQ_FILE_NAME | output file where genomic positions of sequenced reads are stored to | |
| READ_NUMBER | 5,000,000 | number of reads sequenced |
| READ_LENGTH | 36 | length of the reads |
| PAIRED_END | false | switch on/off paired-end reads |
During the "Final Library Preparation" step of the Flux Simulator pipeline the cDNA fragments are optionally filtered by gel segregation, and also optionally amplified by a PCR (i.e., polymerase chain reaction).