Expression | ||
---|---|---|
NB_MOLECULES | 5,000,000 | Number of RNA molecules initially in the experiment |
TSS_MEAN | 50 | Average deviation from the annotated transcription start site (TSS) |
POLYA_SCALE | 100 | Scale of the Weibull distribution, shifts the average length of poly-A tail sizes |
POLYA_SHAPE | 1.5 | Shape of the Weibull distribution describing poly-A tail sizes |
Fragmentation | ||
FRAG_SUBSTRATE | RNA | Specifies RNA as the substrate of fragmentation |
FRAG_METHOD | UR | Uniform random fragmentation |
FRAG_UR_ETA | 350 | Average expected framgent size after fragmentations, i.e., number of breaks per unit length (exhautiveness of fragmentation) |
FRAG_UR_D0 | 1 | Minimum length of fragments produced by UR fragmentation |
FRAG_UR_DELTA | NaN | Geometry of molecules in the UR process depends logarithmically on molecule length |
Reverse Transcription | ||
RTRANSCRIPTION | YES | |