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Parameters
Parameter Name | Default Value | Parameter Range | Description |
|---|---|---|---|
FRAGMENTATION | true | {true,false} | switches fragmentation on/off |
FRAG_METHOD | UR | {NB,UR,EZ} | select the nebulization (NB), uniform-random fragmentation (UR), or enzymatic digestion (EZ) model |
FRAG_SUBSTRATE | RNA | {RNA,DNA} | select whether fragmentation is carried out before (RNA) or after (DNA) reverse transcription |
Overview
| Description | |
|---|---|
LIB_FILE_NAME | the file from which fragments are read, and to which new fragments are written |
Overview
During the "Final Library Preparation" step of the Flux Simulator pipeline the cDNA fragments are optionally filtered by gel segregation, and also optionally amplified by a PCR (i.e., polymerase chain reaction)Timepoint (FRAG_SUBSTRATE) and nature (FRAG_METHOD) of the fragmentation have to be chosen. Specific parameters then are required for each of the simulated fragmentation processes.
Details
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Overview
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