Hi,

 

I just run Flux Capacitor

using parameter.txt:

ANNOTATION_FILE HoSa_build37.2_Genes_genes.gtf
COUNT_ELEMENTS [SPLICE_JUNCTIONS, INTRONS]
ANNOTATION_MAPPING AUTO

 

./flux-capacitor -p parameter.txt -i control.sorted.bam -o control.fluxcapacitor.gtf &

My outputfile contains only 303 lines, and then I got the following error (after about half an hour):

[WARN] Further collapsed transcripts are sent at debug verbosity level.
[ERROR] Error while iterating loci:
java.lang.ArrayIndexOutOfBoundsException: 0
at barna.model.splicegraph.SplicingGraph.constructGraph(SplicingGraph.java:1241)
at barna.flux.capacitor.graph.AnnotationMapper.<init>(AnnotationMapper.java:141)
at barna.flux.capacitor.graph.AnnotationMapper.<init>(AnnotationMapper.java:131)
at barna.flux.capacitor.reconstruction.FluxCapacitor$LocusSolver.call(FluxCapacitor.java:1041)
at barna.flux.capacitor.reconstruction.FluxCapacitor.explore(FluxCapacitor.java:2541)
at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:1782)
at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:73)
at barna.commons.launcher.Flux.main(Flux.java:197)


[ERROR] java.lang.ArrayIndexOutOfBoundsException: 0
java.lang.RuntimeException: java.lang.ArrayIndexOutOfBoundsException: 0
at barna.flux.capacitor.reconstruction.FluxCapacitor.explore(FluxCapacitor.java:2591)
at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:1782)
at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:73)
at barna.commons.launcher.Flux.main(Flux.java:197)
Caused by: java.lang.ArrayIndexOutOfBoundsException: 0
at barna.model.splicegraph.SplicingGraph.constructGraph(SplicingGraph.java:1241)
at barna.flux.capacitor.graph.AnnotationMapper.<init>(AnnotationMapper.java:141)
at barna.flux.capacitor.graph.AnnotationMapper.<init>(AnnotationMapper.java:131)
at barna.flux.capacitor.reconstruction.FluxCapacitor$LocusSolver.call(FluxCapacitor.java:1041)
at barna.flux.capacitor.reconstruction.FluxCapacitor.explore(FluxCapacitor.java:2541)
... 3 more

 

 

what is going wrong??

Thanx

Hanneke 

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1 Comment

  1. Dear Hanneke,

    I created an issue ticket from your observation:

    http://sammeth.net/jira/browse/BARNA-365

    The error occurs when the splicing graph is constructed from the annotation, so I guess that something in the annotation file is not as expected. If possible, could you point us where to find the annotation that provokes the behavior, and please also provide us with the flux version you are using.

    Thanks

    Micha