Child pages
  • Control error model for longer reads and polyA-tales in bed alignements.
Skip to end of metadata
Go to start of metadata
Dear Flux authors/maintainters/developpers,
We (I plus Nicolas Philiipe & Mikaël Salson) have been using FluxSimulator quite a lot recently in order to create a fair benchmark for RNA-Seq mapping softwares, and we must say that it is a really good piece of software!
However we have faced some difficulties in the design of our simulated libraries :
The first difficulty concerns the error model that Flux uses and generalizes for longer reads. In our simulations we want to produce RNA-Seq data with longer reads, as the trend seems to go that way (200bp or greater). But when we do that, the scaled error model generates a lots of errors, and I can understand that. But I think that the errors rate will get lower as technology will evolves. What I am asking you is how could we tune your errors model, to make fewer mistakes (say 1%) on longer reads? I have seen that we can generate custom errors models, but since that data we want to simulate does not already exists this is not a possible alternative. What are your thoughts about that?
The second difficulty concerns the polyA-tales simulations and the aligments reported in the bed file. Some reads does have a part of the polyA-tale and a part that comes from the genome, but the alignement does not mention the polyA-tale part. It is then difficult for us to assess mapping position of those reads. Have I missed something?
Thank you in advance for your answers,
Best regards,
Jérôme Audoux.
  • No labels