When do sequencing, how to fix the insert size and deviation for paired-end reads? If not set, any default value was set? Thanks
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When do sequencing, how to fix the insert size and deviation for paired-end reads? If not set, any default value was set? Thanks
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Thasso Griebel
The insert size of paired-end reads strictly depends on the fragment size and therefor on the fragmentation method you use. In addition, you can apply size selection, which will also effect the insert size.