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The basic problem addressed by the FLUX CAPACITOR.

The exonic structure of two spliceforms (labeled as "SF A" and "SF B") is shown, with aligned reads from by RNAseq methods (top) . Those reads mapped to the edges of a splicing graph (bottom) represent a signal, measured as the FLUX - the relative coverage along an exonic stretch. Where transcripts overlap in exons, their respective flux is combined. Given the information from all edges in a locus, signal separation is achieved by decomposition across a flow