Hello Michael,
Here is a modified extract from my *.pro file. Since my reference transcript length is 1688bp, and the covered fraction as shown, the actual covered length of the transcript comes to 588bp (am I right there?).
locus | transcript_ID | length | expressed fraction | expressed number | sequenced fraction | sequenced number | covered fraction |
---|---|---|---|---|---|---|---|
Chr1:3631-5899W | AT1G01010.1 | 1688 | 2.0002340273812036E-6 | 10 | 2.6374959613343093E-6 | 8 | 0.348341226577759 |
My read headers from the given transcript are:
Code Block |
---|
@Chr1:3631-5899W:AT1G01010.1:2:1688:10:276/1 @Chr1:3631-5899W:AT1G01010.1:2:1688:10:276/2 @Chr1:3631-5899W:AT1G01010.1:3:1688:1134:1342/1 @Chr1:3631-5899W:AT1G01010.1:3:1688:1134:1342/2 @Chr1:3631-5899W:AT1G01010.1:4:1688:886:1144/1 @Chr1:3631-5899W:AT1G01010.1:4:1688:886:1144/2 @Chr1:3631-5899W:AT1G01010.1:5:1688:1340:1686/1 @Chr1:3631-5899W:AT1G01010.1:5:1688:1340:1686/2 |
My questions are:
Thanks for your time and help.