Date: Fri, 29 Mar 2024 02:46:55 +0100 (CET) Message-ID: <997999320.2819.1711676815192@localhost> Subject: Exported From Confluence MIME-Version: 1.0 Content-Type: multipart/related; boundary="----=_Part_2818_1049460488.1711676815190" ------=_Part_2818_1049460488.1711676815190 Content-Type: text/html; charset=UTF-8 Content-Transfer-Encoding: quoted-printable Content-Location: file:///C:/exported.html
I use flux-simulator-1.2.2-20140614022955, since in this ve= rsion you have fixed the problem with the short length transcripts (<3 n= t).
Now the program crashes at library preparation step
[LIBRARY] Reverse Transcription
[LIBRARY] Configuration
Mode: RH
PWM: default
RT MIN: 500
RT MAX: 5500
Processing Fragments * FAILED
[ERROR] Error while fragmenting : null
java.lang.NullPointerException
at barna.flux.simulator.fragmentation.Fragmenter.toCDF(Fragmenter.java:788)=
at barna.flux.simulator.fragmentation.FragmentReverseTranscription.process=
(FragmentReverseTranscription.java:226)
at barna.flux.simulator.fragmentation.Fragmenter.process(Fragmenter.java:596=
)
at barna.flux.simulator.fragmentation.Fragmenter.call(Fragmenter.java:222)=
at barna.flux.simulator.SimulationPipeline.call(SimulationPipeline.java:440)=
at barna.flux.simulator.SimulationPipeline.call(SimulationPipeline.java:54=
)
at barna.commons.launcher.Flux.main(Flux.java:197)
Could you please help?
Gleb