Recently I am using AStalavista to do a alternative splicing project with our own genome. And I got one question based on the software.
I have download the newest version of AStalavista which is V3.01, However I feel a bit strong because the result produced by software is different between run AStalavista locally on your own computer and run it on your web server (http://genome.crg.es/astalavista/) (with the same gtf file). When I run it locally, it showed 7092 AS event has been detected, but based on the result produced by the web server, the AS event is more than 8000.
I don't know what happened, that's why I want your help. the command I used on my own computer is
>bash astalavista -t asta -i gtf_file
I didn't attached the gtf file I intend to use, as it is oversized, but if u need it, I can gzip it and send to you. Just let me know.
Thanks a lot!