simulated by in silico library preparation and sequencing. Our models do not simulate molecular processes on the level of atoms, but represent a collection of deterministic approaches with a minimal set of parameters that can be estimated from observations of the experiment that is to be reproduced. Model development has to be understood as an iterative process to a maximal convergence of "model world" and "real world". It always requires a careful evaluation of all hypotheses and assumptions by comparison with experimental data. |