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We interpret G with each edge

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where f_{i} represents a factor that expresses the fraction of the respective transcript expression t_{i} observed between tail_{e} and head_{e}. In the trivial case, f_{i} corresponds to the proportion of the interval [tail_{e}; head_{e}] in comparison to the entire length of the processed transcript. The correction factorÂ

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The crux of the flux is that an RNASeq experiment provides a series of observations on the underlying expression level t_{i} along the transcript body. Following tradition in transportation problems, we model all of these observations as a system of linear equations by inferring Equation 1 on all

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Solving the linear program (Eq.2) imposed by a locus intrinsically provides an estimate for the expression level t_{i} of all alternative transcripts that are annotated. 
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