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The Profile (.PRO) format is designed to describe the simulated characteristics of each transcript from the reference annotation, one per line. After each step of a simulation run, tab-separated are added to the file. |
Column NumberNr | Name | Value | Description | ||
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1 | LOCUS_IDLocus | chrom:start-end[W|C] | identifier for of the intrinsic splicing transcriptional locus, given by the chromosome (chrom), start and respectively end position, and the strand (Watson or Crick). | ||
2 | TRANSCRIPTTranscript_ID | String | transcript identifier from the reference annotation. | ||
3 | CODINGCoding | [CDS|NC] | specifies whether the transcript has an annotated coding sequence (CDS) or not (NC) | ||
4 | LENGTHLength | Integer | the spliced mature length of the transcript molecule after splicing out introns, disregarding the poly-A tail, as annotated in the reference annotation | ||
5RFREQ | _EXPExpressed Fraction | Float | relative frequency fraction of RNA copies of this transcript after simulated expressionmolecules that represent transcripts that are qualitatively equal to this RNA form | ||
6AFREQ | _EXPExpressed Number | Integer | absolute number of expressed RNA molecules | ||
7RFREQ | _LIBLibrary Fraction | Float | relative frequency fraction of cDNA molecules derived in the final library that have been produced from this transcript after library construction | ||
Library Number | 8 | AFREQ_LIB | Integer | absolute number of cDNA molecules fragments generated from this transcript | |
9RFREQ | _SEQSequenced Fraction | Float | relative frequency fraction of total reads that have been sequenced from this transcript | ||
10AFREQ | _SEQSequenced Number | Integer | absolute number of reads sequenced from this transcript |
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