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changes.mady.by.user bogdan mirauta

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Thank you,

Bogdan

5bias.png

 

 

 

PARAMETER TABLE:

...

Code Block
titleParameters
collapsetrue
NB_MOLECULES

...

TSS_MEAN    NaN

POLYA_SCALE    50

POLYA_SHAPE     2

 

## Fragmentation

FRAG_SUBSTRATE  RNA

FRAG_METHOD     UR

     50000
TSS_MEAN    NaN
POLYA_SCALE    50
POLYA_SHAPE     2


## Fragmentation
FRAG_SUBSTRATE  RNA
FRAG_METHOD     UR
FRAG_NB_LAMBDA

...

 50


## RT parameters
RTRANSCRIPTION YES
RT_PRIMER      RH
RT_LOSSLESS    YES
RT_MIN        2000
RT_MAX        6000


## PCR / Filtering
## no [GC] bias to not observe sequence based biases


PCR_DISTRIBUTION  default
PCR_PROBABILITY 0.5
GC_MEAN NaN
FILTERING         YES
SIZE_SAMPLING     MH


# Sequencing
FASTA    true
READ_NUMBER     1000000
READ_LENGTH     50
PAIRED_END      NO

 

 

 

## RT parameters

RTRANSCRIPTION YES

RT_PRIMER      RH

RT_LOSSLESS    YES

RT_MIN        2000

RT_MAX        6000

 

## PCR / Filtering

## no [GC] bias to not observe sequence based biases

 

PCR_DISTRIBUTION  default

PCR_PROBABILITY 0.5

GC_MEAN NaN

FILTERING         YES

SIZE_SAMPLING     MH

 

# Sequencing

FASTA    true

READ_NUMBER     1000000

READ_LENGTH     50

PAIRED_END      NO