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The read sequence output is a multi-FASTA file, where each fasta block contains a description line that starts with a ">" ("greater than") symbol and the following one or multiple lines containing the read sequence. If a quality/error-model is provided, the very related FASTQ file format is produced, where the ">" identifier is replaced by the "@" symbol, and a quality block is following the fasta block, which uses a "+" separator and subsequently provides the qualities of the read sequences. The description line contains the read identifier as described in the Sequencing Section. |
Example
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FASTQ
| Code Block |
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@chr1:4847775-4887990W:NM_001159750:1:2668:917:1137/1
AAGAGATGAGGAAAAACCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGA
+
IEEIIGIIIIIIF<GGEEHHHD4<D@147=;7*+BDBGACDGGHIIIIIHHDGGDB@@FEGGD9DGIHHHIH@BDG
@chr1:4847775-4887990W:NM_001159750:1:2668:917:1137/2
CCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTACATACAACAAATGTTGTCATTGGTTCA
+
G:GB78??:9>>;?EGGGGHIDGDD=EBFGIIIHHGGGIIIIHHIIIIHHIGEIIIIIHIFCBFIHGD@@@BBEIC |
FASTA
| Code Block |
|---|
>chr1:4847775-4887990W:NM_001159750:1:2668:917:1137/1
AAGAGATGAGGAAAAACCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGA
>chr1:4847775-4887990W:NM_001159750:1:2668:917:1137/2
CCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTACATACAACAAATGTTGTCATTGGTTCA |
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