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Parameters
Parameter Name | Default Value | Parameter Range | Description |
|---|---|---|---|
| RTRANSCRIPTION | true | {true,false} | switch reverse transcription on/off |
| RT_PRIMER | RH | {PDT,RH} | chooses random (RH) or poly-dT primers for first strand synthesis |
| RT_MOTIF | sequence motif that | ||
| RT_MIN | 500 | >0 | the minimum stretch that is polymerized by the reverse transcriptase enzyme |
| RT_MAX | 5,500 | >0 | the maximum stretch that is polymerized by the reverse transcriptase enzyme (template-fidelity) |
Algorithm
| Section |
|---|
Input: RNA polymers annotated by their start end end coordinates on the transcript sequence they originate from (LIB_FILE) and parameters for reverse transcription. |
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Overview
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