We interpret *G* with each edge

=20

(**Equation 1**)

where *f*_{i} represents a factor that expresses=
the fraction of the respective transcript expression *t*_{i<=
/em>}* observed between tail_{e} and head=
_{e}. In the trivial case, f_{i<=
/sub> corresponds to the proportion of the interval [tail=
e; heade] in comparison to the enti=
re length of the processed transcript. The correction factor
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The crux of the flux is that an RNA-Seq experiment provides a series of =
observations on the underlying expression level *t*_{i along the transcript body. Following tradition in transportation probl=
ems, we model all of these observations as a system of linear equations by =
inferring Equation 1 on all
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=20

(**Equation 2**)

Solving the linear program (Eq.2) imposed by a locus intrinsically provi=
des an estimate for the expression level *t*_{i} of=
all alternative transcripts that are annotated.