Date: Thu, 28 Mar 2024 18:19:09 +0100 (CET) Message-ID: <1208685311.2785.1711646349665@localhost> Subject: Exported From Confluence MIME-Version: 1.0 Content-Type: multipart/related; boundary="----=_Part_2784_1786983631.1711646349663" ------=_Part_2784_1786983631.1711646349663 Content-Type: text/html; charset=UTF-8 Content-Transfer-Encoding: quoted-printable Content-Location: file:///C:/exported.html
Hello,
I was wondering of why there is such a strong and pronounced bias toward= s the 3' end of the genes as show below (lots of reads map at the 5' end, n= o reads are present at the 3' end). This image is obtained from the example= .fastq file distributed from the site using a very simple parameter file
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---- p= arameter file ----
REF_FILE_NAME chrI.gtf
GEN_DIR genome
NB_MOLECULES 100000
READ_NUMBER 1000000
EXPRESSION_K 0
# use default 76-bp error model
ERR_FILE 76
# create a fastq file
FASTA YES