Date: Fri, 29 Mar 2024 14:34:04 +0100 (CET) Message-ID: <1147107598.2867.1711719244206@localhost> Subject: Exported From Confluence MIME-Version: 1.0 Content-Type: multipart/related; boundary="----=_Part_2866_1367447738.1711719244204" ------=_Part_2866_1367447738.1711719244204 Content-Type: text/html; charset=UTF-8 Content-Transfer-Encoding: quoted-printable Content-Location: file:///C:/exported.html
I am a newbie here.
I am working on converting genome/gtf data to FASTA file format and usin=
g flux-simulator for that. The demo example from the flux-simulator works w=
ithout any problem.
After that, I downloaded genome and gtf data from =
http://hgdownload.soe.ucsc.edu/downloads.html, https://genome.ucsc.edu/cgi-bin/hgTables?command=3Dstart.
But while running mouse(mm9) gtf, it does not work at all and shows the er=
ror:
[ERROR] Error while loading stats: Field 5 not found
java.lang.IllegalArgumentException: Field 5 not found
I also tried to run flux simulator for "fly" genome.
I got this error:
[INFO] Loading default PCR distribution
preparing transcript sequences =
** ERROR
[ERROR] Error while preparing sequences:
[ERROR] Error while fragmenting : Problems reading sequence chr2L: pos 228=
77860, len 82,
check whether chromosomal sequence exists / has the correct size
java.lang.RuntimeException: Problems reading sequence chr2L: pos 22877860,=
len 82,
Can you please tell how to solve these problems?